Fig. 1

Comparison between ultrasound and macroscopic images of an ovary treated with saline solution (GC) and an ovary with a 20% CaCl₂ injection (G20). Differences between prior to the application (0.40 cm) (a) and after the application of 150 μl of saline solution (0.51 cm) (b) were noticed. However, 15 days after the procedure (0.39 cm) (c) the ovary showed basal measurements. In the macroscopic image after euthanasia (d), the ovary showed normal structure and color. The G20 ovary showed an increase in its measurements when compared to prior to the application (0.32 cm) (e), after the application (0.53 cm) (f) and 30 days after the procedure (0.64 cm) (g). In gross inspection (h) it was possible to identify an ovarian cyst and adhesions between periovarian fat and ovary. *GC = control group (saline solution); G20 = 20% calcium chloride diluted in 95% ethanol group; cm = centimeters