Table 3 Specific primers used for real-time quantitative PCR
Gene | Primer | Accession |
|---|---|---|
β-actin | F 5′-CAGCAAGCAGGAGTACGATG-3′ | NM_173979.3 |
R 5′-AGCCATGCCAATCTCATCTC-3’ | ||
GPBAR1 | F 5′-AGCATCCATCCATCTTGG-3’ | Gene ID: 317756 |
R 5′-GCTTTATTCAGTCAGAGTGGG-3’ | ||
PRKACA | F 5′-GACCGAAGCCTGAGTGACAG-3’ | Gene ID: 282322 |
R 5′-CAGCTATGTACATCCTCGCG-3’ | ||
CREB1 | F 5′-GAGCCATTGATTTGTGCAAAGATG-3’ | Wei et al. (2017) |
F 5′-GCGAGTGGTGAGAAGCGAAGTG-3’ | ||
TNF | F 5′-AAGTAACAAGCCGGTAGCCCA-3’ | NM_173966.3 |
R 5′-CTTCCAGCTTCACACCGTTG-3’ | ||
IL-1β | F 5′-CTCTCACAGGAAATGAACCGAG-3’ | Lahouassa et al. (2007) [45] |
R 5′-CGCTGCAGGGTGGGCGTATCACC-3’ | ||
IL-10 | F 5′-CCAAGCCTTGTCGGAAATGA-3’ | Leutenegger et al. (2000) [46] |
R 5′-GTTCACGTGCTCCTTGATGTCA-3’ |