Fig. 1

Geographical distribution and phylogenetic analysis of PEDV positive samples in China. Phylogenetic analysis of PEDV strains based on 91 S gene sequences identified in this study and 24 reference S gene sequences in Genbank. Multiple nucleotide sequence alignments are performed with ClustalW algorithm using MEGA 6.0 software. A neighbor-joining method based phylogenetic tree is automatically constructed with 1000 bootstrap replicates using MEGA 6.0 software. Labels at branch tips refer to the strain name and GenBank accession number. The black solid dots mark 91 PEDV S sequences detected in this study. Sequences from different genotypes are marked with different color. GI-a, GI-b, S-INDEL like strain, GII-a, and GII-b are labelled with purple, blue, green, orange, and grey, respectively