Pilot study on CHCF1 genotype in a pig challenge model for enterotoxigenic Escherichia coli F4ab/ac associated post-weaning diarrhea

Table 1 Clinical and microbiological outcomes according to CHCF1 genotype: Combined results for trial 1 and 2

CHCF1 genotype	RS	RR	p-value
Pigs with ETEC diarrhea [n/n_group]	6/6	0/26	< 0.0001
Pigs with diarrhea [n/n_group]	6/6	4/26	0.0001
Mean weight gain (SD) [gram]	391 (356)	707 (464)	0.10
Mean challenge strain shedding (SD) [days]	4.8 (0.7)	1.3 (1.2)	0.0001

Data presented includes combined results from pigs of inoculation groups of trial 1 and 2. RS: heterozygous susceptible, n = 6 pigs. RR: homozygous resistant, n = 26 pigs. Diarrhea = fecal score ≥ 3. ETEC diarrhea = fecal score ≥ 3 with isolation of ETEC. Fecal score (firm and shaped (1), soft and shaped (2), loose (3), watery (4). p-values were generated using Kruskal–Wallis test for analysis of group differences in weight gain and days of challenge strain shedding. Difference in proportions of animals with diarrhea in study period analyzed with firth’s logistic regression. Significantly higher occurrence of ETEC diarrhea and diarrhea was found in RS pigs (coefficient (coef) = 6.53, 95% Confidence interval (CI) = 3.40;12.30 and coef = 4.17, 95% CI = 1.78;9.11, respectively) and more days of challenge strain shedding (χ2 = 13.95, df = 1)

ISSN: 1746-6148