Fig. 3
Representative photomicrographs for liver (A-D), kidney (E–H), thymus (I-L), bursa of Fabricius (M-P) and spleen (Q-T) of broiler chickens treated with different levels of SiO2@AgNPs for 35 days; CD45 immunostaining; bar = 50 μm. Chickens from control group (A, E, I, M, Q); chicken treated with 8 mg of SiO2@AgNPs (B, F, J, N, R); chicken treated with 16 mg of SiO2@AgNPs (C, G, K, O, S); chicken treated with 20 mg of SiO2@AgNPs (D, H, L, P, T) showed: positive immune expression in form of frequent individual infiltration of leukocytes between hepatocytes (A); multifocal areas of mononuclear leukocytic aggregations (arrow) (B); positive immunoexpression of the periportal leukocytic aggregates (arrows) (C, D); negative immune expression against CD45 (E); infrequent interlobular positively stained leukocytes (arrows) (F–H); positive immune staining against CD45 in medullary thymocytes (I); mild reduction in the immunostaining of medullary thymocytes (J); marked reduced intensity of immune expression of medullary thymocytes (K, L); mild to moderate immunoexpression of CD45 in the periphery of bursal follicular (M, N); marked CD45 expression within the atrophied follicle (O) and follicles with cystic structure (astrisk) (P); marked immunoexpression of CD45 at the periarterial lymphoid sheath (PALS) (Q, R); mild to moderate CD45 immunoexpression within PALS (S, T)