Validation of an automated immunoturbidimetric assay for feline serum amyloid A

BMC Veterinary Research

Table 2 Measured SAA and observed/expected ratios following addition of interfering substances to feline samples with low-moderate and moderate-high SAA concentrations

Interferent	Interferent Concentration	Measured SAA (mg/L) Low-Moderate	O/E (%)^b	Measured SAA (mg/L) Moderate-High	O/E (%)^b
Haemoglobin	Blank	18.37	100	49.83	100
	0.146 g/L	18.67	103.02	51.33	103.02
	1.46 g/L	18.28	101.28	50.47	101.28
	14.6 g/L	17.2	96.35	48.01	96.35
Triglyceride	Blank	18.37	100	49.83	100
	2.74 mmol/L	17.95	97.7	53.71	107.81
	4.91 mmol/L	19.44	105.81	51.27	102.91
	9.57 mmol/L	17.4	94.7	51.41	103.19
Bilirubin	Blank	18.37	100	49.83	100
	27 μmol/L	24.57	133.74^a	52.42	105.2
	46 μmol/L	22.4	121.92^a	52.69	105.75
	96 μmol/L	19.61	106.77	50.11	100.57

^aIndicates bias above acceptable limit of ±10% of the blank measurement. ^b Observed/Expected (%)

ISSN: 1746-6148