Fig. 4

Absolute quantification of miRNAs sensitive to haemolysis in whole serum (WS) and serum-derived extracellular vesicles (EV) groups, in patients’ sera with different degrees of haemolysis (validation experiments). Grey columns in the background depict increasing levels of haemolysis, measured using the haemoglobin absorbance at 414 nm. miRNA concentration was measured by means of absolute quantification, converting Ct values into fM miRNA based on miRNA-specific standard curves. Experiments were performed in triplicate and markers show the mean ± SD (bars)