A novel strategy for developing vaccine candidate against Jaagsiekte sheep retrovirus from the envelope and gag proteins: an in-silico approach

Table 1 A list of the eight predicted B cell epitopes from the envelope and gag proteins. The antigenicity, allergenicity, and toxicity of epitopes were assessed

Protein	Predicted epitopes	Start	APCPred prediction score	Vaxijen^a antigenicity
Envelope protein	GTKYGDVGVTGF^b	278	0.61	1.7111
	HISPQQANISFY^b	120	0.66	1.6667
	EQVQSINFRMKI^b	451	0.64	1.6045
	QYPMCFSYQSQH^b	136	0.62	1.402
	GLTTQYPMCFSY^b	132	0.6	1.3312
	ISGIDEKTGKKS^b	164	0.68	1.2867
	HLSIGIGIDTPW^b	191	0.65	1.2565
	YPRVTISGIDEK^b	159	0.59	1.2504
Gag protein	TDTQLNFLPGAY^b	361	0.65	1.3888
	LNFLPGAYAQIS^b	365	0.73	1.2633
	QEKGALTSKDEL^b	165	0.84	1.1569
	PPPPPSLKMHPS^b	123	0.6	1.103
	DWKQTARACLSG^b	308	0.61	1.0534
	FKQLKELKIACS^b	272	0.54	0.8964
	NRQQGIQTSYEM^b	340	0.52	0.8518
	SSTKTEDLSKVR^b	389	0.65	0.824

^aThe threshold for the Vaxijen antigenicity was 0.4
^bRepresents the epitopes inter in the structure of the vaccine construct. All the predicted epitopes were shown to be non-allergen and non-toxic

ISSN: 1746-6148