Fig. 5From: K205R specific nanobody-horseradish peroxidase fusions as reagents of competitive ELISA to detect African swine fever virus serum antibodiesDetermination of optimal reaction conditions of the cELISA. AÂ cELISA analysis of the optimal dilution ratio of pig serum. BÂ Determination of optimal competition time for K205R protein binding between ASFV antibody-positive serum and Nb1-HRP fusion protein. CÂ Optimal colorimetric reaction time of the developed cELISABack to article page