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Fig. 4 | BMC Veterinary Research

Fig. 4

From: K205R specific nanobody-horseradish peroxidase fusions as reagents of competitive ELISA to detect African swine fever virus serum antibodies

Fig. 4

Secretory expression and functional analysis of Nbs-HRP in HEK-293 T cell culture supernatants. A Schematic representation of Nbs-HRP fusion protein construction. B IFA identification of Nbs-HRP expression in HEK-293 T cells using anti-HA monoclonal antibody. C Detection of secretory expression of Nbs-HRP fusion protein in HEK-293 T cell culture supernatants. D Direct ELISA analysis of Nbs-HRP specifically reacted with K205R protein. ASFV DP96R was used as a control. E Titration of the Nbs-HRP binding with the K205R protein. F cELISA comparative analysis of Nb1, Nb35 and Nb82 blocking capability of the binding of ASFV antibody-positive serum to K205R protein

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BMC Veterinary Research

ISSN: 1746-6148

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