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Fig. 1 | BMC Veterinary Research

Fig. 1

From: K205R specific nanobody-horseradish peroxidase fusions as reagents of competitive ELISA to detect African swine fever virus serum antibodies

Fig. 1

Expression and purification of K205R-His protein. A The K205R-His protein was analyzed by SDS-PAGE. B and C Western blotting and iELISA analysis of the antigenic property of the K205R protein using inactivated ASFV antibody-positive serum as the primary antibody. Lane 1: pET30a empty vector control; Lane 2: IPTG, 0.5 mM; Lane 3: Supernatant soluble K205R protein; Lane 4: Inclusion body of K205R protein; Lane 5: Purified K205R protein

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BMC Veterinary Research

ISSN: 1746-6148

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