Fig. 1

Epidemiological survey of Jack Russell Terriers. A PCR-RFLP assay. Representative result of polyacrylamide gel electrophoresis of MseI-digested PCR products. Synthetic wild-type and mutant DNA and DNA samples of previously detected carrier and non-carrier JRTs are used as controls. Case nos. JRT317 and JRT321 are determined to be APC variant carriers based on the presence of bands at 51 and 57 bp derived from APC variant allele. WT, wild-type; MT, mutant type; N, non-carrier; C, carrier. Full-length gel image is provided in Supplementary Fig. 1A. B PCR-direct sequencing. Representative result of a carrier JRT, case no. JRT221. DNA sequence of codons 153–156 in the APC gene determined by PCR-direct sequencing. The red arrows indicate 2-bp substitution at codons 154 and 155. See also Supplementary Fig. 2A. C Endoscopic appearance of polyps developed in antrum of the stomach in an APC variant carrier, JRT152. D, E Histopathology of biopsied samples from adenocarcinoma in the gastric antrum of JRT152. E is higher magnification of (D). Bars = 200 µm (D), 50 µm (E)