Fig. 2
From: Serological investigation of Gyrovirus homsa1 infections in chickens in China
Development of indirect ELISA for anti-GyH1 antibody detection. a Optimal concentration of coating antigen and detection antibody. b Optimal coating antigen solution. c Optimal blocking concentration. d Optimal incubation time. e Detect the optimal activation time of the antibody. f HRP-labeled goat anti-chicken optimal dilution. g The optimal activation time of HRP-labeled goat anti-chicken. h The best time for color development. i ELISA cut-off value. j i-ELISA sensitivity test. k Specific detection by ELISA. l ELISA repeatability test