Fig. 4

Ellagic acid reduces liver injury in hepatic ischemia–reperfusion mice by inhibiting pyroptosis. A, B and C The hepatocellular function in serum samples was evaluated by detecting ALT, AST, and LDH levels (n = 3). D Representative images of hematoxylin and eosin (HE) staining, and representative images of caspase-1 staining, the area of Caspase-1-positive cells in the livers were also determined (scale bar, 100 μm) (n = 6). E and F IL-1β and IL-18 were measured with ELISA in liver samples with Hepatic IRI, sham intervention and EA treatment mice (n = 6). G Caspase-1 activities were measured in liver tissues with Hepatic IRI, sham intervention and EA treatment mice (n = 6). H Examination of the proteolytic cleavage of Caspase-1 and GSDMD in mice liver specimens with hepatic IRI or sham intervention, using immunoblotting analysis. GSDMD-FL, full-length GSDMD; GSDMD-N, the N-terminal cleavage product of GSDMD (n = 6). All data are shown as the mean ± SEM. ****P < 0.0001, ***P < 0.001, **P < 0.01 and *P < 0.05