Table 2 Primers used for RT-qPCR
From: Lipid droplet dynamics in healthy and pyometra-affected canine endometrium
Gene symbol | Gene name | NCBI accession number | Oligo sequence (5′ - 3′) | Amplicon length (bp) | PCR efficiency (%) | R2 value | Reference |
|---|---|---|---|---|---|---|---|
ACACA | Acetyl-CoA carboxylase 1 | XM_005624775.4, XM_005624776.4, XM_005624777.4, XM_038608922.1, XM_038608909.1, XM_005624778.4, XM_005624783.4, XM_548250.7 | F: CGAGATTTCACTGTAGCTTCTCC R: TCATAAGACCATCGACGGATAGA | 137 | 87 | 0.999 | – |
FABP4 | Fatty acid binding protein 4 | XM_038441472.1, XM_038516042.1, XM_038643854.1, XM_038579607.1, XM_845069.6 | F: AGTTACTGCGGATGACAGAA R: ACGCCTTTCATGATACATTCC | 150 | 105 | 0.998 | – |
FASN | Fatty acid synthase | XM_038675370.1, XM_038675367.1, XM_038675369.1 | F: AGGAGTTCTGGGCCAATCTC R: GAGTGACGCTGGGTTGATG | 242 | 93 | 0.998 | – |
OAZ1 | Ornithine decarboxylase antizyme 1 | NM_001127234.1 | F: CTGCTGTAGTAACCTGGGTC R: ACATTCAGCCGATTATCAGAGTA | 145 | 97 | 0.994 | Gabriel et al., 2016 |
PLIN1 | Perilipin 1 | XM_038661340.1 | F: GTACCCTCCTGAGAAGATTG R: GGGCACACTGATGCTATT | 85 | 98 | 0.993 | – |
PLIN2 | Perilipin 2 | XM_005626663.3, XM_003639380.4 | F: AATTTGCCAGAAAGAATGTGCAT R: TCCACCCAGGAGAGGTAGAACTT | 79 | 99 | 0.991 | Â |
PLIN3 | Perilipin 3 | XM_038429023.1, XM_038429022.1 | F: GGGTCAGGAGAAACTACAC R: GTCTCCACCTCTGGTTTG | 93 | 97 | 0.995 | – |
RPL27 | Ribosomal protein L27 | NM_001003102.2 | F: ACTACAATCACCTCATGCCC R: CTTGTACCTCTCCTCGAACTTG | 143 | 94 | 0.998 | Gabriel et al., 2016 |
RPL32 | Ribosomal protein L32 | NM_001252169.1 | F: TGGCCATCAGAGTCACCAATC R: GACGCGCACATAAGCTGTTTAT | 74 | 94 | 0.998 | – |
RPL8 | Ribosomal protein L8 | XM_853403.4 | F: TCTTCCGCCAACAGAGCC R: CTTTGCCTTGTACTTGTGGTAAGC | 102 | 94 | 0.995 | – |
SNAP23 | Synaptosome-associated protein 23 | XM_038442089.1, XM_038442090.1, XM_038442091.1, XM_038442087.1, XM_038442088.1 | F: TGGATAATCTGTCACCAGAAGAAA R: TCCTGAGACTCAATGGCTAAAC | 106 | 90 | 0.999 | – |