Fig. 3

Healthy (A) and pyometra (B) resin semi-thin sections stained with PPD. Light brown stained lipid droplets are seen in the surface epithelium of the normal endometrium. In contrast, dark stained lipid droplets were found in the glandular structures (A). Lipid droplets in pyometra samples showed predominantly dark staining in all endometrial epithelia (B). TEM micrographs of healthy glandular (C) and surface (E) endometrial epithelium. Only a limited number of lipid droplets was seen in the glands (arrows). Pyometra sections of surface epithelium (D, F) had a higher incidence of lipid droplets and lipid droplets were larger. Fusion of lipid droplets was frequently observed in pyometra samples (arrow) (F). Note, that the lipid droplets differ in their inner structures. Scale bars (A, B) = 20 μm, (C, D, F) = 2500 nm, (E) = 1000 nm. (G) Results of measurement of lipid droplets in TEM ultrathin sections. LD size was increased in pyometra surface and glandular epithelium compared to healthy surface and glandular epithelium