Utility of ultra-rapid real-time PCR for detection and prevalence of Rickettsia spp. in ticks

BMC Veterinary Research

Table 2 Primers used for detection and sequencing of Rickettsia spp. from ticks

Primer name	Sequence (5′-3′)	Target gene (bp)	Cycling conditions	Reference
ITS-F	GATAGGTCGGGTGTGGAAG	ITS, 388	50 cycles, 95 °C (4 s) -64 °C (4 s)-72 °C (4 s)	[49]
ITS-R	TCGGGATGGGATCGTGTG	ITS, 388	50 cycles, 95 °C (4 s) -64 °C (4 s)-72 °C (4 s)	[49]
Rr17k. 1p	TTTACAAAATTCTAAAAACCAT	17 kDa protein antigen, 539	35 cycles, 95 °C (30 s)-47 °C (30 s)-72 °C (1 min)	[50]
Rr17k. 539n	TCAATTCACAACTTGCCATT	17 kDa protein antigen, 539	35 cycles, 95 °C (30 s)-47 °C (30 s)-72 °C (1 min)
Rr17k. 90p	GCTCTTGCAACTTCTATGTT	17 kDa protein antigen, 450	35 cycles, 95 °C (30 s)-52 °C (30 s)-72 °C (1 min)
Rr17k. 539n	TCAATTCACAACTTGCCATT	17 kDa protein antigen, 450	35 cycles, 95 °C (30 s)-52 °C (30 s)-72 °C (1 min)
Rr190k. 71p	TGGCGAATATTTCTCCAAAA	ompA, 650	35 cycles, 95 °C (30 s)-49 °C (30 s)-72 °C (1 min)	[50]
Rr190k. 720n	TGCATTTGTATTACCTATTGT	ompA, 650	35 cycles, 95 °C (30 s)-49 °C (30 s)-72 °C (1 min)	[50]
Rr190k. 71p	TGGCGAATATTTCTCCAAAA	ompA, 532	35 cycles, 95 °C (30 s)-52 °C (30 s)-72 °C (1 min)	[50]
Rr190k. 602n	AGTGCAGCATTCGCTCCCCCT	ompA, 532	35 cycles, 95 °C (30 s)-52 °C (30 s)-72 °C (1 min)	[51]

ISSN: 1746-6148