Brucella spp. distribution, hosting ruminants from Greece, applying various molecular identification techniques

Katsiolis, Aristomenis; Papadopoulos, Dimitrios K.; Giantsis, Ioannis A.; Papageorgiou, Konstantinos; Zdragas, Antonis; Giadinis, Nektarios D.; Petridou, Evanthia

doi:10.1186/s12917-022-03295-4

BMC Veterinary Research

Table 2 Primers used for Brucella identification

From: Brucella spp. distribution, hosting ruminants from Greece, applying various molecular identification techniques

Primer Name	Primer sequence (5΄-3΄)	Size of product (bp)^a	Annealing temperature	Reference
Bruce06F	ATGGGATGTGGTAGGGTAATCG	274, 408, 542^a	51^oC	Le Flèche et al. [30]
Bruce06R	GCGTGACAATCGACTTTTTGTC	274, 408, 542^a	51^oC	Le Flèche et al. [30]
Bruce08F	ATTATTCGCAGGCTCGTGATTC	330, 348, 366^a	51^oC	Le Flèche et al. [30]
Bruce08R	ACAGAAGGTTTTCCAGCTCGTC	330, 348, 366^a	51^oC	Le Flèche et al. [30]
Bruce11F	CTGTTGATCTGACCTTGCAACC	509, 257, 383^a	51^oC	Le Flèche et al. [30]
Bruce11R	CCAGACAACAACCTACGTCCTG	509, 257, 383^a	51^oC	Le Flèche et al. [30]
Bruce12F	CGGTAAATCAATTGTCCCATGA	345, 392, 375^a	51^oC	Le Flèche et al. [30]
Bruce12R	GCCCAAGTTCAACAGGAGTTTC	345, 392, 375^a	51^oC	Le Flèche et al. [30]
Bruce42F	CATCGCCTCAACTATACCGTCA	538, 539, 289^a	51^oC	Le Flèche et al. [30]
Bruce42R	ACCGCAAAATTTACGCATCG	538, 539, 289^a	51^oC	Le Flèche et al. [30]
Bruce43F	TCTCAAGCCCGATATGGAGAAT	170, 182, 182^a	51^oC	Le Flèche et al. [30]
Bruce43R	TATTTTCCGCCTGCCCATAAAC	170, 182, 182^a	51^oC	Le Flèche et al. [30]
Bruce45F	ATCCTTGCCTCTCCCTACCAG	187, 151, 151^a	51^oC	Le Flèche et al. [30]
Bruce45R	CGGGTAAATATCAATGGCTTGG	187, 151, 151^a	51^oC	Le Flèche et al. [30]
Bruce55F	TCAGGCTGTTTCGTCATGTCTT	234, 273, 273^a	51^oC	Le Flèche et al. [30]
Bruce55R	AATCTGGCGTTCGAGTTGTTCT	234, 273, 273^a	51^oC	Le Flèche et al. [30]
27F	AGAGTTTGATCMTGGCTCAG	1412	50^oC	Frank et al. [53]
1492R	TACGGY TACCTTGTTACGACTT	1412	50^oC	Frank et al. [53]
BP26F	GCCCCTGACATAACCCGCTT	1029	58^oC	Gupta et al. [54]
BP26R	GAGCGTGACATTTGCCGATA	1029	58^oC	Gupta et al. [54]
OMP31F	TGACAGACTTTTTCGCCGAA	720	55^oC	Vizcaino et al. [55]
OMP31R	TATGGATTGCAGCACCGC	720	55^oC	Vizcaino et al. [55]

^aProducts are size-specific for B. suis, B. melitensis and B. abortus, respectively

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