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Table 2 PCR primers used for amplification of the full-length VP2 gene of FPV and CPV

From: Molecular characteristics and genetic evolutionary analyses of circulating parvoviruses derived from cats in Beijing

Primer

Sequence 5’ to 3’

Positiona

Length (bp)a

F1F

CCACCTCATATTTTCATCAA

2709–2728

1325

F1R

TGAATCCAATCTCCTTCTG

4015–4033

 

F2F

GATGAAAATCAAGCAGCAG

3885–3903

817

F2R

CCTTCTAAATCCTATATCAAATAC

4678–4701

 
  1. aThe size and nucleotide position of the PCR products, according to the genomic sequence of CPV-2 (GenBank accession no. M38245)