Table 2 Results of BVD antibody ELISAs performed by the participating laboratories. The ELISA format (competitive or indirect) is given in brackets following the name and manufacturer of the test.
test system | 01/21 (positive, anti-BVDV-2) | 02/21 (negative) | 03/21 (positive anti-BVDV-1) | 04/21 (negative) | 05/21 (positive, anti-BDV) | 06/21 (positive) | 07/21 (positive) | 08/21 (positive) | 09/21 (negative) | 10/21 (positive) | |
|---|---|---|---|---|---|---|---|---|---|---|---|
Monoscreen AbELISA BVDV (E0)/blocking, Bio-X Diagnostics S.A. (competitive) | no. tests | 2 | 2 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 |
no. positive | 2 | 0 | 2 | 0 | 2 | ||||||
no. doubtful | 0 | 0 | 0 | 0 | 0 | ||||||
no. negative | 0 | 2 | 0 | 2 | 0 | ||||||
Monoscreen AbELISA BVDV (NS3)/blocking, Bio-X Diagnostics S.A. (competitive) | no. tests | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
no. positive | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | |
no. doubtful | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
no. negative | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | |
ID Screen® BVD p80 Antibody Competition, Innovative Diagnostics (competitive) | no. tests | 27 | 27 | 27 | 27 | 27 | 22 | 22 | 22 | 22 | 22 |
no. positive | 27 | 0 | 0 | 0 | 27 | 21 | 18 | 19 | 1 | 18 | |
no. doubtful | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | |
no. negative | 0 | 27 | 27 | 27 | 0 | 1 | 3 | 3 | 21 | 3 | |
BVDV Total Ab Test, IDEXX (indirect) | no. tests | 24 | 24 | 24 | 24 | 24 | 16 | 16 | 16 | 16 | 16 |
no. positive | 24 | 0 | 24 | 0 | 24 | 16* | 0 | 0 | 0 | 0 | |
no. doubtful | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
no. negative | 0 | 24 | 0 | 24 | 0 | 0 | 16 | 16 | 16 | 16 | |
BVDV p80 Ab Test, IDEXX (competitive) | no. tests | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
no. positive | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | |
no. doubtful | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | |
no. negative | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | |
Svanovir® BVDV-Ab Screening, SVANOVA (indirect) | no. tests | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
no. positive | 3 | 0 | 3 | 0 | 3 | 3 | 3 | 1 | 0 | 2 | |
no. doubtful | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
no. negative | 0 | 3 | 0 | 3 | 0 | 0 | 0 | 2 | 3 | 1 | |
Svanovir® BVDV-Ab Confirmation, SVANOVA (indirect) | no. tests | 8 | 8 | 8 | 8 | 8 | 9 | 9 | 9 | 9 | 9 |
no. positive | 8 | 0 | 8 | 0 | 8 | 9 | 8 | 3 | 0 | 4 | |
no. doubtful | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
no. negative | 0 | 8 | 0 | 8 | 0 | 0 | 1 | 6 | 9 | 5 | |
PrioCheckTM Ruminant BVD p80 Ab Serum & Milk Kit, Thermo Fisher Scientific (competitive) | no. tests | 3 | 3 | 3 | 3 | 3 | 2 | 2 | 2 | 2 | 2 |
no. positive | 3 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 1 | |
no. doubtful | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
no. negative | 0 | 3 | 3 | 3 | 0 | 1 | 2 | 2 | 2 | 1 | |
PrioCheckTM Bovine BVDV Ab Plate Kit, Thermo Fisher Scientific (competitive) | no. tests | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
no. positive | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | |
no. doubtful | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
no. negative | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | |
in-house (unknown) | no. tests | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
no. positive | 1 | 0 | 1 | 0 | 1 | ||||||
no. doubtful | 0 | 0 | 0 | 0 | 0 | ||||||
no. negative | 0 | 1 | 0 | 1 | 0 |