Fig. 1From: Establishment of enzyme-linked immunosorbent assays based on recombinant S1 and its truncated proteins for detection of PEDV IgA antibodyThe construction of S1T1, S1T2 and S1T3 recombinant balculovirus. AÂ Division strategy of PEDV S1 gene into three segments as S1T1, S1T2 and S1T3. BÂ Construction strategy of S1T1, S1T2 and S1T3 recombinant balculovirus plasmids. CÂ Identification of recombinant balculovirus plasmids by double digestion identification with target fragments of 4827Â bp (pFASTBac plasmid), 924Â bp (S1T1), 927Â bp (S1T2) and 894Â bp (S1T3). DÂ Identification of the recombinant bacmids by PCR with expected products respectivelyBack to article page