Table 2 Phenotypic characterisation of biofilm production by MPA and genotypic detection by PCR of ica, bhp, aap and embp genes from 125 ovine and 70 human NAS isolates
OVINE Isolates (n) | Biofilm production (MPA) | Biofilm genes | ||||||||||||||
PIA | Proteinaceous factors | |||||||||||||||
WBPa | BPb | icaA | icaD | icaA/D | bhp | aap | embp | |||||||||
n | % | n | % | n | % | n | % | n | % | n | % | n | % | n | % | |
S. epi (57) | 17 | 30 | 11 | 19 | 1 | 1.7 | 1 | 1.7 | 1 | 1.7 | 30 | 52.6 | 20 | 16 | 54 | 94.7 |
S. chr (29) | 0 | 0 | 1 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 13.8 | 0 | 0 | 8 | 27.5 |
S. hae (17) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 11.7 | 1 | 5.8 |
S. sim (8) | 7 | 87.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
S. cpr (6) | 3 | 50 | 0 | 0 | 1 | 16.6 | 0 | 0 | 0 | 0 | 1 | 16.6 | 0 | 0 | 0 | 0 |
S. war (5) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 40 | 0 | 0 | 0 | 0 |
S. sap (1) | 1 | 100 | 0 | 0 | 1 | 100 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
S. int (1) | 1 | 100 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
S. mus (1) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Total (125) | 29 | 23.2 | 12 | 9.6 | 3 | 2.4 | 1 | 0.8 | 1 | 0.8 | 37 | 29.6 | 22 | 17.6 | 63 | 50.4 |
HUMAN Isolates (n) | Biofilm production (MPA) | Biofilm genes | ||||||||||||||
PIA | Proteinaceous factors | |||||||||||||||
WBPa | BPb | icaA | icaD | icaA/D | bhp | aap | embp | |||||||||
n | % | n | % | n | % | n | % | n | % | n | % | n | % | n | % | |
S. hae (28) | 1 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 14.3 | 0 | 0 |
S. epi (26) | 9 | 34.6 | 0 | 0 | 10 | 38.5 | 10 | 38.5 | 10 | 38.5 | 16 | 61.5 | 25 | 96.1 | 23 | 88.5 |
S. lug (4) | 3 | 75 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
S. hom (4) | 4 | 100 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 100 | 0 | 0 |
S. cap (3) | 3 | 100 | 0 | 0 | 3 | 100 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
S. war (2) | 2 | 100 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 50 | 1 | 50 |
S. xyl (1) | 1 | 100 | 0 | 0 | 1 | 100 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
S. pas (1) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
S. sap (1) | 1 | 100 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Total (70) | 24 | 34.3 | 0 | 0 | 14 | 20 | 10 | 14.3 | 10 | 14.3 | 16 | 22.8 | 34 | 48.6 | 24 | 34.3 |