Combined effects of dietary Laminaria digitata with alginate lyase on plasma metabolites and hepatic lipid, pigment and mineral composition of broilers

Table 4 Effect of experimental diets on hepatic vitamin E homologues and pigments from broilers

Item	Control	LA	LAR	LAE	SEM	p-value
*Diterpene profile, μg/g*
α-Tocopherol	22.3^a	18.7^ab	15.2^b	20.3^ab	1.63	0.030
γ-Tocopherol	0.526^a	0.281^b	0.175^b	0.267^b	0.0414	< 0.001
*Pigments, μg/100 g*
β-Carotene	22.0^b	40.3^a	40.8^a	42.6^a	0.04	< 0.001
Chlorophyll-a¹	9.34^b	14.3^ab	17.0^ab	20.0^a	2.52	0.033
Chlorophyll-b²	16.8	22.1	26.6	31.7	4.22	0.096
Total chlorophylls³	26.1	36.4	43.6	51.7	6.72	0.065
Total carotenoids⁴	170^b	227^a	208^ab	222^a	10.0	0.001
Total chlorophylls+carotenoids⁵	196^b	263^a	251^a	273^a	13.2	0.001

The broilers were fed: 1) a corn-soybean basal diet (Control); 2) the basal diet plus 15% of L. digitata (LA); 3) the basal diet plus 15% of L. digitata supplemented with 0.005% of Rovabio® Excel AP (LAR); and 4) the basal diet plus 15% of L. digitata supplemented with 0.01% of recombinant CAZyme (LAE)
SEM standard error of the mean
¹Ca = 11.24 × A₆₆₂–2.04 × A₆₄₅
²Cb = 0.13 × A₆₄₅–4.19 × A₆₆₂
³Ca + b = 7.05 × A₆₆₂ + 18.09 × A₆₄₅
⁴Cx + c = (1000 × A₄₇₀–1.90 × Ca - 63.14 × Cb) / 214
⁵(Ca + b) + (Cx + c)
^a.b,cDifferent superscripts within a row indicate a significant difference (p < 0.05)

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