Fig. 8
From: NADH oxidase of Mycoplasma hyopneumoniae functions as a potential mediator of virulence
The apoptosis of hTERT-PBECs induced by rNOX and different virulent Mhp strains was assessed by flow cytometry using the annexin-V-FITC/PI double staining method 12 h post-infection. A: Negative control hTERT-PBECs cultured for 12 h in DMEM/F12 medium without FBS and growth factors before double staining; rNOX, the apoptosis rate of recombinant rNOX protein-infected hTERT-PBECs 12 h post-infection before double staining. B: Highly virulent Mhp strain 168 infected with hTERT-PBECs for 12 h treated without and with rNOX polyclonal antibody. C: Low virulent Mhp strain 168L infected with hTERT-PBECs for 12 h treated without and with rNOX polyclonal antibody