Fig. 5
From: Molecular cloning, characterization, and functional analysis of the uncharacterized C11orf96 gene
The mRNA and protein expression levels of Felis catus C11orf96 in different tissues. A-C The dynamic range of qPCR for C11orf96 quantification in the kidney, liver, spleen, lung, intestine, heart, and trachea. The trachea was used as a reference for data analysis. The β-actin acted as an internal reference gene. The trachea served as the analytical reference. All data are obtained from three independent experiments. D The protein expression level in different tissues was determined by western blotting assay. GAPDH was used as the housekeeping gene control. Rabbit polyclonal antibody for C11orf96 was prepared in our laboratory