Fig. 1

Protein identification in bronchoalveolar lavage fluid (BALF) of West Highland white terriers (WHWTs). For each sample, protein identification was performed on BALF from healthy control (n = 6) and CIPF (n = 6) WHWTs and is based on tryptic peptides. a Box plots compare the total protein concentration between healthy (purple) and CIPF (orange) WHWT BALF. Top and bottom of the box represent the 75% (Q3) and 25% (Q1) percentile, the line inside the box locates the median and whiskers define 1.5 times the interquartile range. b A 12% SDS-PAGE entire gel stained with Coomassie Brilliant Blue dye highlights protein bands in the control (purple) and CIPF (orange) WHWT BALF (10µL of each BALF sample). The band marked with an asterisk was subjected to in-gel digestion, confirming its identity as albumin. c Number of positive protein matches identified per sample based on tryptic peptides (summarised in d). Searches were completed through Proteome Discoverer/MASCOT at 1% FDR, based on one of more peptides for identification