Detection of chicken DNA in commercial dog foods

BMC Veterinary Research

Table 1 Concentrations of chicken DNA in tested foods

Item	Cт Mean	Cт SD	Quantity Mean	Quantity SD	RSD%
D1	18.87	0.24	5.15	0.88	17.14
D2	21.38	0.13	0.89	0.08	9.08
D3	17.46	0.16	13.65	1.50	11.01
D4	21.42	0.05	0.87	0.03	3.83
D5	23.67	0.19	0.18	0.02	13.14
D6	21.55	0.07	0.79	0.04	5.08
D7	20.19	0.05	2.04	0.07	3.24
D8	27.68	0.24	0.01	0.00
W1	27.07	0.17	0.02	0.00
W2	29.80	0.08	0.00	0.00
W3	34.84	1.39	0.00	0.00
W4	28.49	0.34	0.01	0.00
W5	30.49	0.15	0.00	0.00
W6	18.65	0.13	5.96	0.54	8.98
W7	21.91	2.81	0.20	0.03	16.62
NTC	36.12	0.14	0.00	0.00
PTC	22.54	0.01	0.40	0.00

Cт Mean – average of Cт, where Cт – threshold cycle, Cт SD – Cт standard deviation, Quantity Mean—average chicken DNA concentration value for three independent DNA isolations, Quantity SD—average standard deviation of chicken DNA concentration for three independent DNA isolations, RSD %—relative standard deviation of chicken DNA concentration for three independent DNA isolations, NTC negative control, PTC positive control

ISSN: 1746-6148