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Table 2 Changes in the canine sperm velocity ratios (%) during cold storage (5 °C) in the extenders containing different levels of melatonin versus the controls

From: Supplementation of melatonin to cooling and freezing extenders improves canine spermatozoa quality measures

Sampling Days

Group

Negative Control

Positive Control

M1

M2

M3

STR1

74.03 ± 3.16 1b

67.57 ± 2.15 12a

74.08 ± 1.95 1ab

72.74 ± 2.16 1ab

71.12 ± 1.55 1ab

STR3

73.90 ± 3.08 12bc

66.51 ± 1.40 12a

72.17 ± 1.86 12ac

72.78 ± 2.20 1c

70.58 ± 1.18 1ac

STR5

67.90 ± 1.21 23

67.15 ± 1.96 2

64.81 ± 2.14 2

67.52 ± 1.04 23

69.24 ± 1.31 1

STR7

63.63 ± 1.19 3

61.88 ± 1.54 13

62.50 ± .83 23

62.84 ± 1.36 3

62.60 ± 1.05 23

STR9

52.44 ± .86 4

59.60 ± 2.18 3

55.13 ± 2.19 3

62.82 ± 1.58 3

54.01 ± 5.12 3

LIN1

41.89 ± 3.53 12

36.27 ± 3.26 12

42.59 ± 4.18 1

40.33 ± 2.62 12

38.04 ± 2.61 1

LIN3

40.88 ± 2.85 2bc

33.24 ± 1.42 12a

37.97 ± 1.86 1ac

39.77 ± 2.12 2c

36.41 ± 1.66 1ac

LIN5

32.82 ± .76 13

33.35 ± 1.48 2

31.23 ± 1.53 123

33.74 ± 1.05 134

34.29 ± 1.35 2

LIN7

30.52 ± .81 13

28.53 ± 1.39 3

29.12 ± .49 3

29.13 ± 1.07 34

30.00 ± .52 13

LIN9

21.56 ± 1.80 4ab

26.87 ± 1.45 34abc

24.12 ± 2.58 34ab

31.12 ± 1.80 4c

24.06 ± 2.62 3b

WOB1

56.30 ± 2.43 12

53.29 ± 3.01 12

57.15 ± 4.36 1

55.24 ± 1.93 1

53.28 ± 2.49 1

WOB3

55.12 ± 1.56 1b

49.91 ± 1.41 124a

52.49 ± 1.22 12ab

54.51 ± 1.24 12ab

51.50 ± 1.62 1ab

WOB5

48.31 ± .33 234

49.59 ± .89 2

48.08 ± .77 2

49.92 ± .85 13

49.45 ± 1.05 1

WOB7

47.94 ± .38 34

46.02 ± 1.29 134

46.59 ± .42 23

46.30 ± .80 14

47.94 ± .34 12

WOB9

41.02 ± 2.82 4

45.06 ± 1.41 4

43.55 ± 3.21 3

49.46 ± 1.60 234

44.50 ± 1.97 2

  1. The different numbers and letters in superscripts indicate a statistically significant difference (P ≤ 0.05) within and between columns, respectively. A two-way repeated-measures ANOVA statistical test was used to make a comparison between treatment groups at different times. Pairwise comparisons with LSD confidence interval adjustments were carried out to examine the simple effects for the time and study groups. Negative Control (without Melatonin or DMSO), Positive Control (containing DMSO), M1 (containing 0.5 mM Melatonin), M2 (containing 1 mM Melatonin), and M3 (containing 2 mM Melatonin). All numbers are presented as Means±SEM. STR Straightness, LIN Linearity, and WOB Wobble
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BMC Veterinary Research

ISSN: 1746-6148

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