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Table 1 Changes in the canine sperm velocity parameters (μm/s) during cold storage (5 °C) in the extenders containing different levels of melatonin versus the controls

From: Supplementation of melatonin to cooling and freezing extenders improves canine spermatozoa quality measures

Sampling Days

Group

Negative Control

Positive Control

M1

M2

M3

VCL1

70.36 ± 4.40 1

66.42 ± 6.11 1

67.15 ± 4.21 1

65.84 ± 5.36 1

68.72 ± 8.44 12

VCL3

65.55 ± 4.75 1

62.50 ± 6.67 1

63.88 ± 4.47 1

63.53 ± 5.16 1

67.04 ± 8.42 1

VCL5

53.22 ± 6.49 2

52.22 ± 4.44 1

53.33 ± 1.38 1

52.59 ± 2.26 1

53.10 ± 5.01 2

VCL7

23.08 ± 2.14 2

31.05 ± 5.51 2

28.84 ± 2.70 2

34.18 ± 3.65 2

27.19 ± 3.29 3

VCL9

3.19 ± .62 3a

4.66 ± .69 2a

6.55 ± 1.73 3abc

9.57 ± 1.99 3bc

6.06 ± 1.22 4ac

VAP1

39.59 ± 3.05 1

35.56 ± 4.06 1

38.51 ± 4.17 1

36.45 ± 3.39 12

36.40 ± 4.39 12

VAP3

35.91 ± 1.60 1

31.32 ± 3.70 1

33.48 ± 2.22 1

34.56 ± 2.60 2

34.24 ± 3.95 2

VAP5

25.64 ± 2.99 2

25.83 ± 2.09 1

25.63 ± .62 1

26.28 ± 1.31 1

26.14 ± 2.23 1

VAP7

11.04 ± .95 23

14.36 ± 2.64 2

13.41 ± 1.19 2

15.94 ± 1.96 3

13.00 ± 1.48 3

VAP9

1.34 ± .33 3

2.09 ± .26 3

2.92 ± .88 3

4.77 ± 1.06 4

2.74 ± .63 4

VSL1

29.37 ± 2.94 1

24.29 ± 3.37 123

28.70 ± 3.61 1

26.64 ± 2.94 12

25.91 ± 3.23 1

VSL3

26.39 ± .08 1b

20.86 ± 2.57 12a

24.23 ± 1.90 12ab

25.14 ± 1.94 1ab

24.08 ± 2.61 1ab

VSL5

17.27 ± 1.75 2

17.32 ± 1.44 12

16.63 ± .81 2

17.78 ± 1.10 2

18.09 ± 1.54 1

VSL7

6.99 ± .50 23

8.96 ± 1.77 3

8.37 ± .73 3

10.10 ± 1.41 3

8.11 ± .88 3

VSL9

.71 ± .18 3a

1.25 ± .18 4a

1.65 ± .53 4acb

3.02 ± .71 4c

1.51 ± .41 4ab

  1. The different numbers and letters in superscripts indicate a statistically significant difference (P ≤ 0.05) within and between columns, respectively. A two-way repeated-measures ANOVA statistical test was used to make a comparison between treatment groups at different times. Pairwise comparisons with LSD confidence interval adjustments were carried out to examine the simple effects for the time and study groups. Negative Control (without Melatonin or DMSO), Positive Control (containing DMSO), M1 (containing 0.5 mM Melatonin), M2 (containing 1 mM Melatonin), and M3 (containing 2 mM Melatonin). All numbers are presented as Means±SEM. VCL Velocity Curvilinear, VAP Velocity Average Path, and VSL Velocity Straight Path
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BMC Veterinary Research

ISSN: 1746-6148

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