Fig. 8

Plasma membrane functional integrity of the canine post-thaw sperm evaluated by HOS test after cryopreservation in extenders containing different levels of melatonin versus the controls. The One-way ANOVA statistical test was used to make a comparison between treatment groups and the LSD posthoc test was used to perform pairwise comparisons. Different letters in bar superscripts indicate a statistically significant difference (P ≤ 0.05). Negative Control (without Melatonin or DMSO), Positive Control (containing DMSO), M1 (containing 0.5 mM Melatonin), M2 (containing 1 mM Melatonin), and M3 (containing 2 mM Melatonin). All numbers are presented as Mean% ± SEM, HOS=Hypo-Osmotic Swelling Test