Development of a one-step multiplex qRT–PCR assay for the detection of African swine fever virus, classical swine fever virus and atypical porcine pestivirus

Table 2 Repeatability analysis of the multiplex qRT-PCR

Plasmid	Concentration (copies/μL)	Ct values of intra-assay			Ct value of inter-asssay
Plasmid	Concentration (copies/μL)	\(\overline X\)	SD	CV (%)	\(\overline X\)	SD	CV (%)
p-ASFV	2.52 × 10³	27.847	0.370	1.329	27.942	0.379	1.356
	2.52 × 10⁵	20.738	0.168	0.810	20.747	0.207	0.998
	2.52 × 10⁷	13.845	0.166	1.199	13.725	0.124	0.903
P-CSFV	2.52 × 10³	28.001	0.153	0.546	27.946	0.186	0.666
	2.52 × 10⁵	20.359	0.200	0.982	20.457	0.239	1.168
	2.52 × 10⁷	13.109	0.156	1.190	13.076	0.088	0.673
P-APPV	2.52 × 10³	27.328	0.152	0.556	27.314	0.137	0.502
	2.52 × 10⁵	19.125	0.267	1.396	19.128	0.207	1.082
	2.52 × 10⁷	12.348	0.145	1.174	12.302	0.157	1.276

ISSN: 1746-6148