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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem cells in horses

Fig. 4

Results of tri-linage differentiation. Synovial membrane-derived mesenchymal stem cells (SM-MSCs) were cultured under osteogenic induction and stained with alizarin red. Scale bar = 1 mm (A). SM-MSCs induced to chondrocytes were stained with Alcian blue. Scale bar = 500 μm (B). Adipogenic induction of SM-MSCs was evaluated by staining with oil red O. Scale bar = 250 μm (C). The negative controls were cultured with incomplete culture medium during the corresponding periods of time taken to induce osteogenic (D), chondrogenic (E), and adipogenic differentiation (F)

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