Table 3 List of PCR primer sequences
Marker | Gene | Primer sequence (5′–3′) | Amplification Size (bp) |
|---|---|---|---|
Housekeeping | GAPDH | F - GGAATCCACTGGCGTCTTCA R - GGTTCACGCCCATCACAAAC | 122 |
Stemness | Nanog | F - GAATAACCCGAATTGGAGCAG R - AGCGATTCCTCTTCACAGTTG | 141 |
Sox2 | F - AACCCCAAGATGCACAACTC R - CGGGGCCGGTATTTATAATC | 152 | |
MSC markers | CD45 | F - CTCACGCACACAGGCTCGCA R - CCCACCCACTGGCACTGCTG | 159 |
CD90 | F - CAGAACACCTCATGGCTGCTGT R - GGAGAAACCAGACAGAAGCGA | 329 | |
CD105 | F - GGTTCACTGCATCAACATGG R - AAGCTGAAGCGCACATCACC | 278 | |
SOX9 | F - GCTCGCAGTACGACTACACT R - GTTCATGTAGGTGAAGGTGG | 101 | |
COL2A | F - GAAACTCTGCCACCCTGAAT R- GCTCCACCAGTTCTTCTTGG | 145 |