Skip to main content
Fig. 1 | BMC Veterinary Research

Fig. 1

From: Protective immunity induced by oral vaccination with a recombinant Lactococcus lactis vaccine against H5Nx in chickens

Fig. 1

Expression of the HA1-M2 fusion protein in L. lactis. a Schematic diagram of L. lactis/pNZ8149-HA1-M2. b Western blot analysis. M: Western blot marker; Lane 1: Cell culture supernatants of L. lactis/pNZ8149-HA1-M2; Lane 2: Cell lysates of L. lactis/pNZ8149-HA1-M2 (approximately 45 kDa); Lane 3: Cell lysates of L. lactis/pNZ8149; Lane 4: Cell culture supernatants of L. lactis/pNZ8149. c Immunofluorescence microscopy assay. L. lactis/pNZ8149 (left) and L. lactis/pNZ8149-HA1-M2 (right) (magnification: 1000×). d Quantification of L. lactis/pNZ8149-HA1-M2 expressing the HA1-M2 fusion protein measured by whole-cell ELISA. A450 values were obtained from three independent experiments. The bar indicates the means ± SDs

Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us