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Table 4 The list of assays used to determine metabolic and hormonal profiles

From: Changes in metabolic and hormonal profiles during transition period in dairy cattle – the role of spexin

Target

Kit name

Sensitivity

Cat. No.

Manufacturer

Spexin

Spexin / NPQ (Human: Mouse: Bovine) - EIA Kit

0–100

ng/ml

EK-023-81

Phoenix Pharmaceuticals: USA

NEFA

NEFA-HR

0.01–4.0 mmol/l

434–91,795

434–91,995

Wako Chemicals: Germany

BHBA

β-Hydroxybutyrate

0.1–4.5 mmol/l

H 7587

Pointe Scientific: USA

Ghrelin (total)

Ghrelin (TOTAL) RIA

100–10,000 pg/mL

GHRA-88HK

Merck Millipore: USA

Progesterone

Progesterone Elisa kit

0-40 ng/ml

DNOV006

NovaTec: Germany

Glucose

Glucose Oxy

10–500 mg/dl

G7519

Pointe Scientific: USA

Insulin

Insulin-Specific RIA

2–200 μU/mL

HI-14 K

Merck Millipore: USA

IGF-I

IGF-1600 ELISA

0–600 ng/mL

EIA-4140

DRG: Germany

Triglycerides

Triglycerides DST

0–1000 mg/dl

T7531

Pointe Scientific: USA

Cholesterol

Cholesterol kit

0–700 mg/dl

C7510

Pointe Scientific: USA

Ghrelin (active)

Ghrelin (ACTIVE) RIA

10–2000 pg/mL

GHRA-88HK

Merck Millipore: USA

Leptin

Multi-Species Leptin RIA

1–50 ng/mL

XL-85 K

Merck Millipore: USA

Corticosterone

Corticosterone Double Antibody RIA Kit

0–1000 ng/ml

SKU 07120103

MP Biomedicals: USA

17β-estradiol

17 beta-Estradiol Elisa kit

0–600 pg/ml

DNOV003

NovaTec: Germany

AST

AST (SGOT) Liquid Reagents

0–500 IU/l

A7560

Pointe Scientific: USA

ALP

Alkaline Phosphatase

0–800 IU/l

A7505

Pointe Scientific: USA

GGTP

Gamma Glutamyl Transferase Reagent Set

0–1000 IU/l

G7571

Pointe Scientific: USA

  1. NEFA nonesterified fatty acid: BHBA β-hydroxybutyrate acid: IGF – I insulin-like growth factor I: AST aspartate transaminase: ALP alkaline phosphatase: GGTP gamma-glutamyl transferase