Establishment of a peptide-based enzyme-linked immunosorbent assay for detecting antibodies against PRRSV M protein

Table 2 Comparison of peptide ELISA, IDEXX PRRSV X3 Ab ELISA, and VN test in detecting PRRSV antibody response in pigs

Antibody detection method	Week post-vaccination (wpv) (positive number/total number)
	0		2		4		6		8
	Vaccination^a	Control^b	Vaccination	Control	Vaccination	Control	Vaccination	Control	Vaccination	Control
M-A110-129 ELISA	0/10	0/10	0/10	0/10	7/10	0/10	10/10	0/10	10/10	0/10
M-A148-174 ELISA	0/10	0/10	1/10	0/10	10/10	0/10	10/10	0/10	10/10	0/10
IDEXX PRRSV X3 Ab ELISA	0/10	0/10	8/10	0/10	10/10	0/10	10/10	0/10	10/10	0/10
VN test	0/1	0/10	0/10	0/10	10/10	0/10	10/10	0/10	10/10	0/10

^aVaccination group was vaccinated with NADC30 like PRRSV inactivated vaccine, and sera were collected on indicated days and tested bypeptide ELISA, IDEXX PRRSV X3 Ab ELISA, and VN test
^bControl group was not treated, and sera were collected on indicated days and tested by peptide ELISA, IDEXX PRRSV X3 Ab ELISA, and VN test

ISSN: 1746-6148