Table 1 Primers used in this study
From: Characterization and reverse genetic establishment of cattle derived Akabane virus in China
Number | Name | Sequences | Located sites (bp) |
|---|---|---|---|
1 | AKAV-S-F | AGTAGTGAACTCCACTATTAACTACGC | 1-27 |
2 | AKAV-S-R | AGTAGTGTGCTCCACTAATTAACTATAAAC | 827-856 |
3 | AKAV-M1-F | AGTAGTGAACTACCACAACAAAATGATT | 1-28 |
4 | AKAV-M1-R | CTTGTATGCAAGCACTAAAAGC | 1409-1432 |
5 | AKAV-M2-F | CTAGATAATTTCACATCTCATTGCC | 1253-1277 |
6 | AKAV-M2-R | TTTACTCTGGAAATAACTGTTGCTTC | 3182-3207 |
7 | AKAV-M3-F | CAAGATTCAAGACAGCTACATAAC | 2830-2853 |
8 | AKAV-M3-R | AGTAGTGTTCTACCACAACAAATAATTATT | 4280-4309 |
9 | AKAV-L1-F | AGTAGTGTACCCCTAAATACAACATAC | 1-27 |
10 | AKAV-L1-R | CATATTTGGCTTTGATAATATCTTGTCAAC | 1399-1428 |
11 | AKAV-L2-F | CGAAGCTATAAAAATTGGTACCTC | 1167-1190 |
12 | AKAV-L2-R | CCATCTCCAGGTTCGCTAATCATCTCATCTG | 2975-3005 |
13 | AKAV-L3-F | CGAGCATATACTCCAAGTCATGAAAAATC | 2808-2836 |
14 | AKAV-L3-R | TCATTCCGTTACGATCCATTTG | 4777-4798 |
15 | AKAV-L4-F | GAAGACCTTTGTTGAGACGTATCGACAG | 4520-4647 |
16 | AKAV-L4-R | GCATCAATTTTGAAAGATCTATACCCCC | 6040-6097 |
17 | AKAV-L5-F | GGATAGAATAGAAATGCTCAATATCG | 5937-5962- |
18 | AKAV-L5-R | AGTAGTGTGCCCCTAAATGCAATAATATAC | 6839-6868 |
19 | rAKAV-S-F | GGGGTACCCGTCTCATATAGAGTAGTGAACTCCACTATTAACTACGC | 1-27 |
20 | rAKAV-S-R | GCTCTAGACGTCTCTACCCAGTAGTGTGCTCCACTAATTAAC | 834-856 |
21 | rAKAV-M-F | GGGGTACCCGTCTCATATAGAGTAGTGAACTACCACAACAAAATG | 1-25 |
22 | rAKAV-M-R | GCTCTAGACGTCTCTACCCAGTAGTGTTCTACCACAACAAATAATT | 4283-4309 |
23 | rAKAV-L-F | GGGGTACCCGTCTCATATAGAGTAGTGTACCCCTAAATACAACATAC | 1-27 |
24 | rAKAV-L-R | GCTCTAGACGTCTCTACCCAGTAGTGTGCCCCTAAATGCAATAATATAC | 6838-6868 |