Table 6 Sequences of oligonucleotide primers and probes used in real-time PCR
Target | Gene target | Forward primer | Reverse primer | Probe | Labels | Reference |
|---|---|---|---|---|---|---|
Genus Brucella | bcsp31 gene | 5’GCTCGGTTGCCAATATCAATGC3’ | 5’GGGTAAAGCGTCGCCAGAAG3’ | 5’AAATCTTCCACCTTGCCCTTGCCATCA3’ | 5’Fluorophore-6-FAM, 3’Quencher BHQ1 | [42] |
B. abortus | alkB gene | 5’GCGGCTTTTCTATCACGGTATTC3’ | 5’CATGCGCTATGATCTGGTTACG3’ | 5’CGCTCATGCTCGCCAGACTTCAATG3’ | 5’HEX 3’BHQ1 | [42] |
B. melitensis | BMEI1162 gene | 5’AACAAGCGGCACCCCTAAAA3’ | 5’CATGCGCTATGATCTGGTTACG3’ | 5’CAGGAGTGTTTCGGCTCAGAATAATCCACA3’ | 5’Texas Red 3’BHQ2 | [42] |