Characterization of porcine sapelovirus prevalent in western Jiangxi, China

BMC Veterinary Research

Table 3 Primers for PSV detection and complete VP1 gene amplification

Primer name	Oligonucleotide sequence (5′-3′)^c	Genome location
PSV-rF^a	CGTGCTCCTTTGGTGATTC	211-229^d
PSV-rR^b	GAAAGAGTAGTAGTAGATTCC	460-480^d
PSV-VP1-I-R	TGTRAANGANCCYCTRTCAAA	3245-3265^d/2788-2808^e/3207-3227^f
PSV-VP1-E-R	CTCCARTTRTTDGCWGCRTGGTAGGG	3299-3324^d/2842-2867^e/3261-3286^f
PSV-VP1-A-I-F	CCACCDGGHGCMCCNTCHACATG	2180-2202^d
PSV-VP1-A-E-F	ACAYTDTCWTAYAAYGGRTGGGT	2126-2148^d
PSV-VP1-B-I-F	AGTGCDKCRGAYAAYTTTGT	1765-1784^e
PSV-VP1-B-E-F	TRTCRTAYAATGGRTGGGT	1673-1691^e
PSV-VP1-C-I-F	ACAGTKAGYGCTKCRGACAACTTTG	2202-2226^f
PSV-VP1-C-E-F	TTCCARCARACWGCACTDGTTGT	2142-2164^f

^a F: forward primer
^b R: reverse primer
^cR, A or G; Y, C or T; W, A or T; N, A, T, G, or C; D, A, G, or T; K, G or T; H, A, C, or T; M, A or C
^d Location of primers relative to the complete genomic sequence of PSV-JD2011 (GenBank # KF539414)
^e Location of primers relative to the complete genomic sequence of PSV-HuN1 (GenBank # KX354740)
^f Location of primers relative to the complete genomic sequence of PSV-V13 (GenBank # AF406813)

ISSN: 1746-6148