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Table 2 Infection rate of EHDV and PALV detected by qRT-PCR and RPA-LFD assay

From: Development and evaluation of recombinase polymerase amplification combined with lateral flow dipstick assays for co-detection of epizootic haemorrhagic disease virus and the Palyam serogroup virus

  qRT-PCR
EHDV PALV EHDV + PALV
Positive Negative Positive Negative Positive Negative
RPA-LFD Positive 22 2 18 1 10 2
Negative 2 70 2 75 3 81
Coincidence rate (22 + 70)/96 × 100 %=95.8 % (18 + 75)/96 × 100 %=96.9 % (10 + 81)/96 × 100 %=94.8 %