Fig. 3

Analytical sensitivity of the RPA-LFD assay in the co-detection of EHDV and PALV. Top: Results of RPA nfo reactions detected by LFD detectors; bottom: results of RPA nfo reaction analyzed by agarose gel electrophoresis. Lane 1–6: a ten-fold serial dilution of purified EHDV and PALV Seg-1 DNA fragments from 105 to 100 copies/µL, which served as templates; lane 7: negative control; lane 8: 100 bp DNA marker