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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Development and evaluation of recombinase polymerase amplification combined with lateral flow dipstick assays for co-detection of epizootic haemorrhagic disease virus and the Palyam serogroup virus

Fig. 2

Specificity of the RPA-LFD assay in the detection of EHDV and PALV. Top: Results of RPA nfo reactions detected by LFD detectors; bottom: results of RPA nfo reaction analyzed by agarose gel electrophoresis. Lane 1–7: cDNAs of EHDV-1, -2, -5, -6, -7, -8 and − 10 strains served as templates; lane 8–10: cDNAs of BCV, CHUV and DAV strains served as templates; lane 11–16: cDNAs of AHS inactivated vaccine strain, BTV-1, -16 strains, GXOV, TIBOV and YUOV served as templates; lane 17: negative control; lane 18: 100 bp DNA marker

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