Fig. 2
![Fig. 2](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12917-021-02977-9/MediaObjects/12917_2021_2977_Fig2_HTML.png)
Specificity of the RPA-LFD assay in the detection of EHDV and PALV. Top: Results of RPA nfo reactions detected by LFD detectors; bottom: results of RPA nfo reaction analyzed by agarose gel electrophoresis. Lane 1–7: cDNAs of EHDV-1, -2, -5, -6, -7, -8 and − 10 strains served as templates; lane 8–10: cDNAs of BCV, CHUV and DAV strains served as templates; lane 11–16: cDNAs of AHS inactivated vaccine strain, BTV-1, -16 strains, GXOV, TIBOV and YUOV served as templates; lane 17: negative control; lane 18: 100 bp DNA marker