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Table 2 Analytical methods for serum biochemistry using spectrophotometry

From: Effect of age, sex, and body size on the blood biochemistry and physiological constants of dogs from 4 wk. to > 52 wk. of age

Analyte Method/Principle Reaction type Reading mode Type of reaction Wavelength
AST Modified IFCC Kinetic Decreasing 340 nm
ALT Modified IFCC Kinetic Decreasing 340 nm
LDH Modified IFCC Kinetic Increasing 340 nm
ALP p-NPP Kinetic Increasing 405 nm
GGT GLUPAC Kinetic Increasing 405 nm
Total protein Biuret Endpoint Monochromatic Increasing 540 nm
Albumin BCG Endpoint Monochromatic Increasing 630 nm
Cholesterol Enzymatic Endpoint Monochromatic Increasing 500 nm
Triglycerides Enzymatic Endpoint Monochromatic Increasing 500 nm
Glucose Enzymatic Endpoint Monochromatic Increasing 500 nm
Urea Urease, GLDH Fixed time Decreasing 340 nm
Creatinine Jaffe, acid picric Kinetic Increasing 510 nm
  1. ALP alkaline phosphatase; ALT alanine aminotransferase; AST aspartate aminotransferase; BCG bromocresol green dye binding; GGT gamma-glutamyl transferase; GLDH glutamate dehydrogenase; GLUPAC Lγ-glutamyl-3-carboxy-4-nitroanilide; IFCC International Federation of Clinical Chemistry; LDH lactate dehydrogenase; p-NPP ρ-nitrofenil phosphate