Table 2 Analytical methods for serum biochemistry using spectrophotometry
Analyte | Method/Principle | Reaction type | Reading mode | Type of reaction | Wavelength |
|---|---|---|---|---|---|
AST | Modified IFCC | Kinetic | – | Decreasing | 340 nm |
ALT | Modified IFCC | Kinetic | – | Decreasing | 340 nm |
LDH | Modified IFCC | Kinetic | – | Increasing | 340 nm |
ALP | p-NPP | Kinetic | – | Increasing | 405 nm |
GGT | GLUPAC | Kinetic | – | Increasing | 405 nm |
Total protein | Biuret | Endpoint | Monochromatic | Increasing | 540 nm |
Albumin | BCG | Endpoint | Monochromatic | Increasing | 630 nm |
Cholesterol | Enzymatic | Endpoint | Monochromatic | Increasing | 500 nm |
Triglycerides | Enzymatic | Endpoint | Monochromatic | Increasing | 500 nm |
Glucose | Enzymatic | Endpoint | Monochromatic | Increasing | 500 nm |
Urea | Urease, GLDH | Fixed time | – | Decreasing | 340 nm |
Creatinine | Jaffe, acid picric | Kinetic | – | Increasing | 510 nm |