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Table 4 Hematology and serum biochemistry of dogs fed diets supplemented with silybin and commercial hepatoprotectant (EXP1)

From: Effects of silybin supplementation on nutrient digestibility, hematological parameters, liver function indices, and liver-specific mi-RNA concentration in dogs

Item CON
(n = 18)
mean
HEP
(n = 18)
mean
SIL
(n = 18)
mean
SEM P
WBC (×  109/L) 10.5a 8.84b 9.02b 0.28 0.02
NEUT (× 109/L) 6.99a 5.51b 5.37b 0.25 0.01
LYM (×  109/L) 2.15 2.50 2.58 0.08 NSD
MONO (× 109/L) 0.90a 0.65b .74ab 0.04 0.03
EOS (× 109/L) 0.45a 0.15b .29ab 0.05 0.03
BASO (× 109/L) 0.04 0.04 .03 0.00 NSD
RBC (×  1012/L) 6.81 6.79 6.92 0.08 NSD
HEM (g/L) 160 164 166 1.74 NSD
HTC (%) 0.46 0.47 0.47 0.01 NSD
MCV (fL/cell) 67.9 68.7 68.5 0.34 NSD
MCH (pg/cell) 23.5b 24.1a 24.0a 0.08 0.001
MCHC (g/L) 346 352 350 1.49 NSD
PLT (× 109/L) 219 245 241 8.82 NSD
Albumin (g/L) 3.7 3.6 31.0 0.31 NSD
ALT (U/L) 37.7 39.0 36.1 1.36 NSD
Alpha-Amylase (U/L) 752a 467b 593b 31.8 <  0.001
ALP (U/L) 42.6a 35.4b 31.2b 1.29 <  0.001
AST (U/L) 28.6 32.6 32.2 0.89 NSD
Total protein (g/L) 58.2 59.6 59.4 0.55 NSD
Total bilirubin (μmol/l) 2.94b 3.39a 3.03ab 0.07 0.04
Cholesterol (mmol/L) 4.28 4.61 4.69 0.12 NSD
CK (U/L) 152 154 154 5.71 NSD
Fructosamine (μmol/l) 242b 265a 258ab 3.32 0.01
GLDH (U/L) 2.98 3.40 3.27 0.09 NSD
GLUC (mmol/L) 6.18a 5.09b 5.42b 0.11 <  0.001
GGT (U/L) 2.30b 4.08a 3.32a 0.20 <  0.001
Creatinine (μmol/l) 71.8 67.5 68.0 1.64 NSD
LDH (U/L) 157 141.1 146 12.3 NSD
Lipase (DGGR) (U/L) 58.8 68.0 76.5 3.41 NSD
Urea (mmol/L) 3.98 4.60 4.48 0.15 NSD
TG (mmol/L) 0.42b 0.59a 0.54a 0.02 <  0.001
Chloride (mmol/L) 114 112 113 0.34 NSD
Inorganic phosphorus (mmol/L) 1.31 1.52 1.52 0.05 NSD
Magnesium (mmol/L) 0.70b 0.79a 0.80a 0.01 <  0.001
Potassium (mmol/L) 4.17b 4.52a 4.54a 0.05 <  0.001
Sodium (mmol/L) 148 146 147 0.37 0.05
Calcium (mmol/L) 2.56a 2.44b 2.50ab 0.02 0.001
Albumin / globulin ratio 1.12 1.07 1.11 0.03 NSD
Globulin (g/L) 27.5 29.0 28.4 0.59 NSD
  1. Abbreviations: CON, control group; HEP, group fed diet supplemented with commercial hepatoprotectant containing silybin; SIL, group fed diet supplemented with pure silybin; WBC, White blood cells; NEUT, neutrophils; LYM, lymphocytes; MONO, monocytes; EOS, eosinophils; BASO, basophils; RBC, red blood cells; HEM, hemoglobin; HTC, hematocrit; MCV, mean corpuscular volume; MCHC, mean corpuscular hemoglobin concentration; PLT, platelets; ALT, alanine aminotransferase; ALP, alkaline phosphatase; AST, aspartate aminotransferase; CK, creatinine kinase; GLDH, glutamate dehydrogenase; GLUC, glucose; GGT, gamma glutamyl transpeptidase; LDH, lactic dehydrogenase; TG, triglycerides; NSD, not statistically different
  2. Superscripts a,b in the same row indicate significant differences between control versus treatment groups (P <  0.05)