Fig. 2
From: Interaction of porcine circovirus-like virus P1 capsid protein with host proteins
Detection of self-activation of the bait protein. The AH109 yeast cells that were cotransformed with pGADT7 and pGBKT7-ORF1, pGADT7-LargeT and pGBKT7-p53 (as a positive control), pGADT7-LargeT and pGBKT7-laminC (as a negative control), were plated on SD-TL and SD-TLHA medium for the auto activation test. Yeast co-transfected with plasmids pGADT7 and pGBKT7-ORF1 cannot grow on SD-TLHA medium and did not turn blue in the β-galactosidase assay, indicating that pGBKT7-ORF1 does not autonomously activate the reporter genes in yeast cells without a prey protein. The images were cropped, and full-length images are included in Supplementary Fig. 2a to 2c