Table 1 Mix Ratio to real – time PCR [15]
Component | Volume in a 10 μl reaction |
|---|---|
SsoAdvanced™ Universal SYBR® Green Supermix | 5 μl |
Primer (F + R) | 1 μl (0.8 μM) |
DNA matrix | 2 μl (0.04–0.015 × 10−4) |
Sterile water | 2 μl |
Component | Volume in a 10 μl reaction |
|---|---|
SsoAdvanced™ Universal SYBR® Green Supermix | 5 μl |
Primer (F + R) | 1 μl (0.8 μM) |
DNA matrix | 2 μl (0.04–0.015 × 10−4) |
Sterile water | 2 μl |