Fig. 3

Timeline divided in weeks representing the study design of the tests performed in chickens (n = 10). Each chicken underwent eight anaesthetic events: two in the weeks four and five for the minimum anaesthetic concentration (MAC) determination of isoflurane and sevoflurane, three during weeks six to eight for testing of the influence on the bispectral index (BIS) and the visual analogue scale (VAS) of NaCl solution (0.25 ml/kg), butorphanol (1 mg/kg) and medetomidine (0.1 mg/kg) under isoflurane anaesthesia and three during weeks 13 to 15 for testing of the influence on BIS and VAS of NaCl solution (0.25 ml/kg), butorphanol (1 mg/kg) and medetomidine (0.1 mg/kg) under sevoflurane anaesthesia