Table 8 Gene specific primer sequences used for RT-qPCR
Gene symbol | Full gene name | GenBank accession number | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|---|---|
ACACA | Acetyl-CoA carboxylase alpha | NM_001114269.1 | ggccatcaaggacttcaacc | acgatgtaagcgccgaactt | 120 |
APOA5 | Apolipoprotein A-V | NM_001159308.1 | agggaaaggcttctgggacta | tgtctttcagtctcgtgggctc | 107 |
CAT | Catalase | NM_214301.2 | agaggaaacgcctgtgtgag | ttgtccagaagagcctgaatg | 133 |
CEBPA | CCAAT/enhancer binding protein (C/EBP) alpha | XM_003127015.2 | ggccagcacacacacattaga | cccccaaagaagagaaccaag | 71 |
ChREBP | MLX interacting protein-like | XM_003481002.2 | tgacatgatccagcctgacc | gggggctcagagaagtttga | 126 |
CPT1A | Carnitine palmitoyltransferase 1A | NM_001129805.1 | cgattatccaccagccagac | caccccataaccatcgtcag | 120 |
CRAT | Carnitine O-acetyltransferase | NM_001113047.1 | ggcccaccgagcctacac | atggcgatggcgtaggag | 138 |
DGAT | Diacylglycerol acyltransferase | NM_214051.1 | caactaccgtggcatcctga | tagaaacagccgtgcattgc | 67 |
FABP1 | Fatty acid binding protein 1 | NM_001004046.1 | aacttctccggcaaataccaa | attctgcacgatttccgatg | 129 |
FADS1 | Fatty acid desaturase 1 | NM_001113041.1 | gtgggtggacttggcctg | gatgtgcatggggatgtggt | 166 |
FADS2 | Fatty acid desaturase 2 | NM_001171750.1 | gccttacaaccaccagcatga | aggccaagtccacccagtc | 122 |
FASN | Fatty acid synthase | NM_001099930.1 | acaccttcgtgctggcctac | atgtcggtgaactgctgcac | 112 |
GPX1 | Glutathione peroxidase 1 | NM_214201.1 | ggagatcctgaattgcctca | gataaacttggggtcggtca | 181 |
GSR | Glutathione-disulfide reductase | XM_003483635.4 | ggtgtgtgccaacaaagagg | aaccctgcagcagcatttcatca | 77 |
HSL | Hormone sensitive lipase | 397,583 | tcgtggctcaactccttcct | gggtgtcctgtgtctcgg | 190 |
LPIN1 | Lipin 1 | NM_001130734.1 | aagtcgccgccctgtatttc | ttgtcgctggcctgttttgt | 67 |
NOS2 | Nitric oxide synthase 2 | NM_001143690.1 | cctggtgccctgctttgt | ctgccagaaactgcggaag | 118 |
NOS3 | Nitric oxide synthase 3 | NM_214295.1 | ggctgcatgacattgagagc | ctcgtcgcggtagagatggt | 98 |
PFKL | Phosphofructokinase liver | 100,621,757 | gctcaaggaggacaccgact | cgccagcatcttcagcat | 85 |
PLIN2 | Perilipin 2 | NM_214200.2 | catgtccggtgctctcccta | cccagtcacagcccctttag | 160 |
PPARA | Peroxisome proliferator-activated receptor alpha | NM_001044526.1 | tttccctctttgtggctgct | ggggtggttggtctgcaag | 128 |
SCD | Stearoyl-CoA desaturase | NM_213781.1 | agccgagaagctggtgatgt | gaagaaaggtggcgacgaac | 140 |
SOD1 | Superoxide dismutase 1 | NM_001190422.1 | gctgtaccagrgcaggtcctc | cacagtggccacaccatctt | 125 |
SOD2 | Superoxide dismutase 2 | NM_214127.2 | gtggaggccacatcaatcat | ccgacagatacagcggtcaa | 148 |
SOD3 | Superoxide dismutase 3 | NM_001078688.1 | accagttcggggacctgag | ggcgaagttgccgaagtct | 104 |
SREBF1 | Sterol regulatory element binding transcription factor 1 | NM_214157.1 | gtgctggcggaggtctatgt | aggaagaagcgggtcagaaag | 86 |
Housekeeping genes | |||||
RPLP0 | Ribosomal phosphoprotein large P0 subunit | NM_001098598.1 | tccaggctttaggcatcacc | ggctcccactttgtctccag | 95 |
RPL27 | Ribosomal protein L27 | NM_001097479.1 | gtactccgtggatattg | aacttgaccttggcct | 102 |