Fig. 1

Immunohistochemistry. a. CD3, adenocarcinoma. A small number of positive cells were found in the lamina propria (arrow). 40x. b. CD3, normal colon. Scattered cells and clusters of cells were seen in the lamina propria (arrow) and the epithelium. 40x. c. CD18, adenocarcinoma. Fewer positive cells (arrow) and weaker signal than in the control. 10x. d. CD18, control. Characteristic distribution of positive cells, scattered in the lamina propria (*) and concentrated below the crypt epithelium (arrows). 10x. e. Ki-67, adenocarcinoma. Numerous cells have strong, nuclear staining (arrows). 40x. f. Ki67, adenoma. Numerous cells have strong, nuclear staining (arrows). 40x. g. β-catenin, adenocarcinoma. Strong staining of neoplastic epithelial cells. Both cytoplasmic (arrows) and nuclear (arrowheads) staining are evident. 40x. h. β-catenin, crypt epithelium from control. Moderate granular staining of cytoplasm only. The signal seems to partially follow the contour of the cell membrane (arrows). 40x