Table 2 The sequences of the primers and probes of the four swine diseases
From: Multiplex and on-site PCR detection of swine diseases based on the microfluidic chip system
The amplification gene. | 5′ → 3′ Sequence |
|---|---|
PRRSV-ORF1b gene | F1: GCCTGACTAAGGAGCAGTGTTTA |
R2: ACCGCTGTCTCAGTAACAACTAAGC | |
P3: CCGCGACCACAGCGGGTCA | |
PCV2-ORF1 gene | F: TGGAGAGCGGGAGTCTGGT |
R: CTTCCAATCACGCTTCTGCAT | |
P: ACCGTTGCAGAGCAGCACCCTGT | |
PEDV-Spike protein gene | F: TCGCAATCTCAGCGTTATGGT |
R: GGTGCTGCCTGTACCAGAGAGA | |
P: TGTGGTGGTGATGGCGAGCACAT | |
PRV-E (gE) gene | F: TTGTGCTGGCGCTGGGCTCCT |
R: CGACGCGATCTACGTGGAC | |
P: CACAGCACGCAGAGCCAGA |